甲状旁腺激素相关肽及其受体信使核糖核酸在人羊膜和绒毛膜-蜕膜中的表达：对分泌和功能的影响

Expression of parathyroid hormone-related peptide and its receptor messenger ribonucleic acid in human amnion and chorion-decidua: implications for secretion and function.

作者信息

Bruns M E, Ferguson J E, Bruns D E, Burton D W, Brandt D W, Jüppner H, Segre G V, Deftos L J

机构信息

Department of Pathology, University of Virginia, Charlottesville, USA.

出版信息

Am J Obstet Gynecol. 1995 Sep;173(3 Pt 1):739-46. doi: 10.1016/0002-9378(95)90333-x.

DOI:10.1016/0002-9378(95)90333-x

PMID:7573236

Abstract

OBJECTIVE

Our purpose was to define the location and packaging of parathyroid hormone-related peptide in amnion-chorion and the potential target tissues for its action in fetal membranes.

STUDY DESIGN

We studied fetal membranes by use of light microscopic immunocytochemistry with three monoclonal antibodies against distinct regions of the parathyroid hormone-related peptide molecule. For electron microscopy immunogold analysis with a monoclonal antibody specific to the 109-141 fragment was used to observe parathyroid hormone-related peptide intracellularly in amnion membrane and in the chorion layers. Multiplex reverse transcriptase-polymerase chain reaction with Southern blotting was used to identify parathyroid hormone/parathyroid hormone-related peptide receptor and control messenger ribonucleic acids in amnion and chorion-decidua.

RESULTS

All monoclonal antibodies revealed immunoreactive parathyroid hormone-related peptide in the amniotic epithelial cells and in some fibroblast-like cells embedded in the extracellular matrix of the amnion. Parathyroid hormone-related peptide was also found in the chorion in fibroblast and trophoblast layers and in decidua. Ultrastructurally immunogold particles were evenly distributed throughout the amniotic epithelial cells and were present in apical microvilli and near the basal membranes. Electron microscopy studies of the chorion cytotrophoblast also showed freely dispersed immunogold particles of parathyroid hormone-related peptide with no packaging in secretory granules. Low to undetectable levels of parathyroid hormone/parathyroid hormone-related peptide receptor messenger ribonucleic acid were found in amnion tissue, whereas abundant receptor messenger ribonucleic acid was found in chorion-decidua.

CONCLUSIONS

These results suggest the presence of a parathyroid hormone-related peptide paracrine system within the human fetal membranes.

摘要

目的

我们的目的是确定甲状旁腺激素相关肽在羊膜 - 绒毛膜中的定位和包装情况，以及其在胎膜中潜在的作用靶组织。

研究设计

我们使用针对甲状旁腺激素相关肽分子不同区域的三种单克隆抗体，通过光学显微镜免疫细胞化学方法研究胎膜。对于电子显微镜免疫金分析，使用针对109 - 141片段的单克隆抗体来观察羊膜和绒毛膜层细胞内的甲状旁腺激素相关肽。采用多重逆转录聚合酶链反应结合Southern印迹法来鉴定羊膜和绒毛膜 - 蜕膜中的甲状旁腺激素/甲状旁腺激素相关肽受体及对照信使核糖核酸。

结果

所有单克隆抗体均显示羊膜上皮细胞以及羊膜细胞外基质中一些成纤维细胞样细胞内有免疫反应性甲状旁腺激素相关肽。在绒毛膜的成纤维细胞、滋养层细胞和蜕膜中也发现了甲状旁腺激素相关肽。超微结构上，免疫金颗粒均匀分布于整个羊膜上皮细胞，存在于顶端微绒毛和基底膜附近。对绒毛膜细胞滋养层的电子显微镜研究也显示甲状旁腺激素相关肽的免疫金颗粒自由分散，无分泌颗粒包装。在羊膜组织中发现甲状旁腺激素/甲状旁腺激素相关肽受体信使核糖核酸水平低至无法检测，而在绒毛膜 - 蜕膜中发现大量受体信使核糖核酸。