Prévosto J M, Béraud B, Cheminel V, Gaillard Y, Mounier C, Chaulet J F
Laboratoire de biochimie, toxicologie et pharmacologie du professeur Blanchot, hôpital d'instruction des Armées Desgenettes, Lyon, France.
Ann Biol Clin (Paris). 1995;53(1-2):29-32.
A reverse-phase high performance liquid chromatographic method with ultraviolet detection is described for the measurement of doxycycline in human plasma and urine. After liquid-solid extraction on a Bond Elut C18 cartridge, doxycycline and demeclocycline (internal standard) are separated on a Novapak C18 column by isocratic elution. The mobile phase consists of acetonitrile-oxalate buffer, pH 2.3 (25:75; v/v). The eluent is monitored with an ultraviolet detector at 355 nm. The lower limit of quantification in plasma is close to 25 ng/ml. No chromatographic interference can be detected from endogenous compounds, tetracycline group antibiotics or antimalarial drugs. The method is accurate and precision is good with inter- and intra-assay relative standard deviations lower than 6.7%. The chromatographic procedure takes 8 minutes and can be used for therapeutic drug monitoring, clinical and pharmacokinetic studies.
本文描述了一种采用紫外检测的反相高效液相色谱法，用于测定人血浆和尿液中的多西环素。在Bond Elut C18柱上进行液固萃取后，多西环素和地美环素（内标）在Novapak C18柱上通过等度洗脱进行分离。流动相由乙腈 - 草酸盐缓冲液（pH 2.3，25:75；v/v）组成。洗脱液用紫外检测器在355 nm处进行监测。血浆中的定量下限接近25 ng/ml。未检测到内源性化合物、四环素类抗生素或抗疟药物的色谱干扰。该方法准确，批内和批间相对标准偏差低于6.7%，精密度良好。色谱过程耗时8分钟，可用于治疗药物监测、临床和药代动力学研究。
