用于绿硫细菌检测的叶绿体蛋白多克隆抗体探针。
Polyclonal antibodies to chlorosome proteins as probes for green sulfur bacteria.
作者信息
Cahill A D, Stolz J F
机构信息
Department of Biological Sciences, Duquesne University, Pittsburgh, Pennsylvania 15282, USA.
出版信息
Appl Environ Microbiol. 1995 Feb;61(2):784-7. doi: 10.1128/aem.61.2.784-787.1995.
Abstract
We found that polyclonal antibodies raised against chlorosome polypeptides from green sulfur bacteria reacted to Chlorobium tepidum, Chlorobium limicola, and Chlorobium phaeobacteroides but not to Chloroflexus aurantiacus. These antibodies successfully labeled only green sulfur species in marine microbial mat samples. Our results suggest that these antibodies may be useful as immunohistochemical probes.
摘要
我们发现，针对绿硫细菌的叶绿体多肽产生的多克隆抗体可与嗜温绿菌、嗜盐绿菌和噬杆绿菌发生反应，但不与橙黄嗜热栖热菌发生反应。这些抗体仅成功标记了海洋微生物垫样本中的绿硫菌。我们的结果表明，这些抗体可能作为免疫组织化学探针有用。
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