López J R, Jovanovic A, Terzic A
Department of Internal Medicine, Mayo Clinic, Mayo Foundation, Rochester, MN 55905, USA.
Biochem Biophys Res Commun. 1995 Sep 25;214(3):781-7. doi: 10.1006/bbrc.1995.2354.
Spontaneous Ca2+ waves were visualized in quiescent cardiomyocytes loaded with the Ca(2+)-sensitive fluorescent probe, Fluo-3, and imaged by laser confocal microscopy. No sarcomere shortening was detected during wave propagation. This type of Ca2+ waves began at the periphery or in a central region of a myocyte and propagated the length of the cell in one or two directions. The average velocity of wave propagation was 32 microns/sec and the estimated concentration of Ca2+ oscillated from 124, at the bottom, to 311 nM, at the pick of the wave. Ca2+ waves were not confined to a single cell but could spread from cell to cell. These results describe a type of spontaneous Ca2+ waves which does not induce a contractile response in cardiomyocytes.
在加载了Ca(2+)敏感荧光探针Fluo-3的静止心肌细胞中观察到自发Ca2+波，并用激光共聚焦显微镜成像。在波传播过程中未检测到肌节缩短。这种类型的Ca2+波始于心肌细胞的周边或中央区域，并沿一个或两个方向传播整个细胞长度。波传播的平均速度为32微米/秒，估计的Ca2+浓度从波谷的124 nM振荡到波峰的311 nM。Ca2+波不限于单个细胞，而是可以在细胞间传播。这些结果描述了一种在心肌细胞中不诱导收缩反应的自发Ca2+波类型。
