Mashima K, Furuya Y
Department of Science, Rikkyo (St. Paul's) University Nishi-Ikeburkuro, Tokyo, Japan.
Biochem Biophys Res Commun. 1995 Sep 25;214(3):856-60. doi: 10.1006/bbrc.1995.2365.
Non-malignant rat liver epithelial cell line BRL was reported to adhere to a substrate by fibronectin. DNA synthesis of these cells was induced by adhesion to a substrate by fibronectin, while DNA synthesis in non-adhered cells was not observed. These results indicated that DNA synthesis in BRL cells is inducible by the cell adhesion signaling. Adhesion-inducible DNA synthesis was strongly inhibited by Herbimycin A. In contrast, vanadate showed the tendency to promote adhesion-inducible DNA synthesis. These results suggest that DNA synthesis caused by the cell adhesion in these cells was regulated by both phosphorylation and dephosphorylation at tyrosine residue.
据报道，非恶性大鼠肝上皮细胞系BRL通过纤连蛋白附着于基质。这些细胞的DNA合成是由纤连蛋白介导的细胞与基质的黏附所诱导的，而未黏附细胞中未观察到DNA合成。这些结果表明，BRL细胞中的DNA合成可由细胞黏附信号诱导。黏附诱导的DNA合成受到赫曲霉素A的强烈抑制。相反，钒酸盐显示出促进黏附诱导的DNA合成的趋势。这些结果表明，这些细胞中由细胞黏附引起的DNA合成受酪氨酸残基的磷酸化和去磷酸化调控。
