Kawamoto S, Inoue Y, Shinozaki Y, Katakura Y, Tachibana H, Shirahata S, Murakami H
Graduate School of Genetic Resources Technology, Kyushu University, Fukuoka, Japan.
Biochem Biophys Res Commun. 1995 Oct 4;215(1):280-5. doi: 10.1006/bbrc.1995.2463.
The class II major histocompatibility complex (MHC) antigens play important roles in T cell activation and are thought to be involved in tumor development. Using anti-class II antibodies with interferon gamma (IFN-gamma), we have here selected class II MHC antigen positive cancer cells from the human lung adenocarcinoma cell line A549, which is originally negative (> 95%) for the antigens. A part of the class II antigen-positive cells presented a flat morphology, which was not observed in the parental A549 cells. Class II antigen expression in these flat cells was IFN-gamma inducible; there was a correlation between the inducibility and phenotypic changes. A class II antigen-inducible flat subline restored the ability of contact inhibition and anchorage-dependent growth as well as lost tumorigenicity in athymic mice.
II类主要组织相容性复合体（MHC）抗原在T细胞激活中起重要作用，并被认为参与肿瘤发展。我们使用抗II类抗体与干扰素γ（IFN-γ），从人肺腺癌细胞系A549中筛选出II类MHC抗原阳性癌细胞，该细胞系最初对这些抗原呈阴性（>95%）。部分II类抗原阳性细胞呈现扁平形态，而亲代A549细胞中未观察到这种形态。这些扁平细胞中的II类抗原表达是IFN-γ诱导性的；诱导性与表型变化之间存在相关性。一个II类抗原可诱导的扁平亚系恢复了接触抑制和锚定依赖性生长的能力，并且在无胸腺小鼠中丧失了致瘤性。
