转化生长因子-β抑制细胞因子介导的人视网膜色素上皮细胞中诱导型一氧化氮合酶mRNA的表达。

Transforming growth factor-beta inhibits the cytokine-mediated expression of the inducible nitric oxide synthase mRNA in human retinal pigment epithelial cells.

作者信息

Kutty R K, Kutty G, Hooks J J, Wiggert B, Nagineni C N

机构信息

Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892-2740, USA.

出版信息

Biochem Biophys Res Commun. 1995 Oct 4;215(1):386-93. doi: 10.1006/bbrc.1995.2477.

DOI:10.1006/bbrc.1995.2477

PMID:7575617

Abstract

Human retinal pigment epithelial (RPE) cells in culture respond to a mixture of cytokines (IFN-gamma, IL-1 beta, TNF-alpha) by producing large amounts of nitric oxide. Transforming growth factor-beta, unlike other growth factors, was found to inhibit this response by more than 75%. The expression of mRNA for the inducible form of nitric oxide synthase in RPE cells treated with cytokines was demonstrated by reverse transcription-polymerase chain reaction, sequencing of the PCR product and northern blotting. Transforming growth factor-beta was highly effective in inhibiting (by 75%) the cytokine-induced nitric oxide synthase mRNA expression. This response by RPE may play an important role in the etiology of infectious and inflammatory diseases affecting retina.

摘要

培养的人视网膜色素上皮（RPE）细胞对细胞因子混合物（γ干扰素、白细胞介素-1β、肿瘤坏死因子-α）产生反应，会产生大量一氧化氮。与其他生长因子不同，转化生长因子-β被发现可抑制这种反应达75%以上。通过逆转录-聚合酶链反应、PCR产物测序和Northern印迹法证实了用细胞因子处理的RPE细胞中诱导型一氧化氮合酶mRNA的表达。转化生长因子-β在抑制（75%）细胞因子诱导的一氧化氮合酶mRNA表达方面非常有效。RPE的这种反应可能在影响视网膜的感染性和炎症性疾病的病因学中起重要作用。