Ryanodine作为人中性粒细胞趋化肽诱导趋化作用的抑制剂。

Ryanodine as inhibitor of chemotactic peptide-induced chemotaxis in human neutrophils.

作者信息

Elferink J G, De Koster B M

机构信息

Department of Medical Biochemistry, University of Leiden, The Netherlands.

出版信息

Biochem Pharmacol. 1995 Sep 28;50(7):975-9. doi: 10.1016/0006-2952(95)00222-l.

DOI:10.1016/0006-2952(95)00222-l

PMID:7575682

Abstract

Ryanodine gave a moderate inhibition of chemotactic peptide-activated chemotaxis by intact human neutrophils. Chemotaxis by electroporated neutrophils was strongly inhibited in the nanomolar concentration range. Inhibition of chemotaxis by electroporated neutrophils occurs at concentrations known to open calcium channels in ryanodine-sensitive Ca2+ stores. Whereas migration by formyl-methionyl-leucyl-phenylalanine (fMLP)- or interleukin-8-activated electroporated neutrophils was strongly inhibited by ryanodine, chemotaxis induced by protein kinase C activators was not affected. This suggests that the importance of ryanodine-sensitive Ca2+ stores for migration depends on the type of activator used. Ryanodine gave an increase of cytoplasmic free calcium due to the liberation of calcium from internal stores and to the influx of extracellular calcium. The results show that the neutrophil contains ryanodine-sensitive calcium stores that might be involved in receptor-mediated chemotaxis.

摘要

雷诺丁对完整的人中性粒细胞趋化肽激活的趋化作用有适度抑制。在纳摩尔浓度范围内，电穿孔中性粒细胞的趋化作用受到强烈抑制。电穿孔中性粒细胞趋化作用的抑制发生在已知能打开雷诺丁敏感Ca2+储存库中钙通道的浓度。虽然甲酰甲硫氨酰亮氨酰苯丙氨酸（fMLP）或白细胞介素8激活的电穿孔中性粒细胞的迁移受到雷诺丁的强烈抑制，但蛋白激酶C激活剂诱导的趋化作用不受影响。这表明雷诺丁敏感Ca2+储存库对迁移的重要性取决于所使用的激活剂类型。由于钙从内部储存库释放以及细胞外钙流入，雷诺丁使细胞质游离钙增加。结果表明，中性粒细胞含有可能参与受体介导趋化作用的雷诺丁敏感钙储存库。