实验性铝中毒在同种异体免疫反应中的作用。

The role of experimental aluminum intoxication in allogeneic immunoresponse.

作者信息

Tzanno-Martins C, Azevedo L S, Tanji M, Tanji M C, Sabbaga E, Marcondes M, Duarte A J

机构信息

Laboratory of Immunogenetics and Experimental Transplantation, University of São Paulo Medical School, Brazil.

出版信息

Transpl Int. 1995;8(5):396-8. doi: 10.1007/BF00337173.

DOI:10.1007/BF00337173

PMID:7576023

Abstract

To evaluate the immunological properties of aluminum (Al) in experimental Al intoxication in rats, we performed heart transplantation and in vitro experiments. Lewis (Lew) rats were intoxicated with intraperitoneal injections of AlCl3. heart transplants were performed using Brown-Norway (BN) rats as donors. Isotransplants and normal Lew were used as controls. No differences in survival were observed. Unidirectional mixed lymphocyte cultures (MLC) and Concanavalin A (Con A)-stimulated cultures were prepared using spleen cells from normal and Al-intoxicated Lew rats. No differences were found in unidirectional MLC. Intoxicated cells showed a less intense response to con A than did normal cells. In conclusion, we could not detect an immunosuppressive role of Al intoxication in experimental cardiac transplantation or in MLC. However, the depressed Con A blastogenic response of Al-intoxicated cells may reflect an immunological role yet to be defined.

摘要

为评估铝（Al）在大鼠实验性铝中毒中的免疫特性，我们进行了心脏移植和体外实验。通过腹腔注射AlCl₃使Lewis（Lew）大鼠中毒。使用Brown-Norway（BN）大鼠作为供体进行心脏移植。同基因移植和正常Lew大鼠用作对照。未观察到存活率有差异。使用来自正常和铝中毒Lew大鼠的脾细胞制备单向混合淋巴细胞培养物（MLC）和刀豆球蛋白A（Con A）刺激的培养物。在单向MLC中未发现差异。中毒细胞对Con A的反应不如正常细胞强烈。总之，我们在实验性心脏移植或MLC中未检测到铝中毒的免疫抑制作用。然而，铝中毒细胞的Con A促有丝分裂反应降低可能反映了尚未明确的免疫作用。