Check J H, Bollendorf A, Askari H A
Department of Obstetrics and Gynecology, University of Medicine and Dentistry of New Jersey, Camden, USA.
Am J Reprod Immunol. 1995 Jul;34(1):20-5. doi: 10.1111/j.1600-0897.1995.tb00915.x.
To determine if the embryo may induce adhesive molecules needed for implantation.
Determination of whether platelet rosetting around lymphocytes might occur when exposed to sera from pregnant, but not nonpregnant patients and from culture fluid from embryos but not oocytes.
90.2% of women with positive sera beta human chorionic gonadotropin (beta-hCG) levels taken at least 12 days postovulation demonstrated platelet rosette factor (PRF) vs only 18.7% when beta-hCG was negative. Using mid-luteal phase sera in women receiving hCG injection 1 wk before, 64.7% had positive PRF when serial beta-hCG levels were positive as did 100% of samples taken from in vitro fertilization (IVF) patients; however, only 15.3% were positive with negative serial hCG levels. Culture media from fertilized oocytes and embryos tested positive for PRF, but follicular fluid and media from unfertilized oocytes were negative.
The early embryo secretes a factor(s) that gains access to maternal serum and promotes increased lymphocyte/platelet adhesiveness.
确定胚胎是否能诱导着床所需的黏附分子。
检测当暴露于怀孕而非未怀孕患者的血清以及胚胎而非卵母细胞的培养液时，淋巴细胞周围是否会出现血小板花环现象。
排卵后至少12天血清β人绒毛膜促性腺激素（β-hCG）水平呈阳性的女性中，90.2%表现出血小板花环因子（PRF），而β-hCG呈阴性时只有18.7%。在提前1周接受hCG注射的女性中使用黄体中期血清，当连续β-hCG水平为阳性时，64.7%的PRF呈阳性，体外受精（IVF）患者采集的样本中100%也是如此；然而，连续hCG水平为阴性时只有15.3%呈阳性。受精卵母细胞和胚胎的培养液PRF检测呈阳性，但卵泡液和未受精卵母细胞的培养液呈阴性。
早期胚胎分泌一种因子，该因子进入母体血清并促进淋巴细胞/血小板黏附性增加。
