N-庚基毒扁豆碱的微生物转化，一种胆碱酯酶的半合成生物碱抑制剂。

Microbial transformation of N-heptyl physostigmine, a semisynthetic alkaloid inhibitor of cholinesterase.

作者信息

So L, White R, Arison B, Petuch B, Schwartz M S, Cheng H, Monaghan R, Chen T S

机构信息

Merck Research Laboratories, Department of Fermentation Microbiology, Rahway, NJ 07065, USA.

出版信息

J Ind Microbiol. 1995 Aug;15(2):108-11. doi: 10.1007/BF01569808.

DOI:10.1007/BF01569808

PMID:7576461

Abstract

The microbiological transformation of N-heptyl physostigmine (L-693,487) (1), a semisynthetic physostigmine cholinesterase inhibitor, was investigated using Verticillium lecanii MF 5713 (ATCC 74148), Acremonium sp MF 5723 (ATCC 74164) and Actinoplanes sp MA 6559 (ATCC 53771). Nine microbial metabolites (2-10) of 1 were isolated and purified using reversed-phase HPLC. The structures of the metabolites were established using spectroscopic techniques including MS and NMR. Some of the microbial metabolites were identical to metabolites present in urine of a dog treated with 1.

摘要

研究了半合成毒扁豆碱胆碱酯酶抑制剂N-庚基毒扁豆碱（L-693,487）（1）在蜡蚧轮枝菌MF 5713（美国典型培养物保藏中心74148）、顶孢霉属MF 5723（美国典型培养物保藏中心74164）和游动放线菌属MA 6559（美国典型培养物保藏中心53771）作用下的微生物转化。使用反相高效液相色谱法分离并纯化了1的9种微生物代谢产物（2 - 10）。通过包括质谱和核磁共振在内的光谱技术确定了代谢产物的结构。一些微生物代谢产物与用1处理的狗尿液中存在的代谢产物相同。