Chikindas M L, Venema K, Ledeboer A M, Venema G, Kok J
Groningen Biomolecular Sciences and Biotechnology Institute, Department of Genetics, University of Groningen, Haren, The Netherlands.
Lett Appl Microbiol. 1995 Sep;21(3):183-9. doi: 10.1111/j.1472-765x.1995.tb01037.x.
Pediocin PA-1 production, immunity and secretion are specified by a cluster of four genes in Pediococcus acidilactici PAC1.0. The production by, secretion of, and immunity to lactococcin A of Lactococcus lactis are also determined by four genes. Here, expression of the pediocin operon in Lactococcus lactis is reported, which could only be achieved by placing it under control of a lactococcal promoter. Expression of the lactococcin A operon in Pediococcus is also described: recombinant clones of Pediococcus were obtained that produced and secreted both active pediocin PA-1 and lactococcin A.
嗜酸乳杆菌PAC1.0中，植物乳杆菌素PA-1的产生、免疫和分泌由四个基因组成的基因簇决定。乳酸乳球菌产生、分泌乳酸乳球菌素A以及对其免疫也由四个基因决定。本文报道了植物乳杆菌素操纵子在乳酸乳球菌中的表达，这只有通过将其置于乳球菌启动子的控制之下才能实现。文中还描述了乳酸乳球菌素A操纵子在嗜酸乳杆菌中的表达：获得了嗜酸乳杆菌的重组克隆，这些克隆可产生并分泌活性植物乳杆菌素PA-1和乳酸乳球菌素A。
