Junge J, Poulsen H, Horn T, Hørding U, Lundvall F
Department of Pathology, Hvidovre Hospital, University of Copenhagen, Denmark.
APMIS. 1995 Jul-Aug;103(7-8):501-10. doi: 10.1111/j.1699-0463.1995.tb01398.x.
Surgical specimens from 62 patients with vulvar dysplasia and carcinoma in situ were morphologically investigated. Lesions were classified according to WHO (mild, moderate, severe dysplasia and carcinoma in situ) and according to Toki et al. (1991) (warty, basaloid, combined warty/basaloid or basaloid/warty types or mixed (warty, basaloid and simple) forms). Following the WHO classification, moderate dysplasia was shown in 4 cases, severe dysplasia in 47 and carcinoma in situ in 11 cases. Pure warty type was shown in 2 cases (both biopsy specimens). One case revealed pure simple dysplasia whereas no case of pure basaloid type was found. Various combinations of warty and basaloid types were shown in 52 cases and mixed forms in 7 cases. The results indicate that pure forms of warty and basaloid types probably do not exist. HPV DNA was detected by PCR in 51/58 cases (88%) (45 with HPV type 16 and 6 with HPV type 33) evenly distributed in all age groups and in all types of lesions (WHO and Toki et al. 1991). By ISH HPV was detected in 24/62 cases (39%) (21 with HPV type 16/18 and 3 with HPV type 31/33), nearly always in warty areas. All these cases were positive for the same virus type by PCR. No case revealed more than one type of HPV. HPV type 6, 11, 18, and 31 were not detected by PCR. The results indicate a correlation between HPV type 16 and 33 and dysplasia/carcinoma in situ in the vulva.
对62例外阴发育异常和原位癌患者的手术标本进行了形态学研究。病变根据世界卫生组织（WHO）分类（轻度、中度、重度发育异常和原位癌）以及根据Toki等人（1991年）的分类（疣状、基底样、疣状/基底样或基底样/疣状联合类型或混合（疣状、基底样和单纯）形式）进行分类。按照WHO分类，4例为中度发育异常，47例为重度发育异常，11例为原位癌。2例（均为活检标本）显示为纯疣状类型。1例显示为纯单纯发育异常，而未发现纯基底样类型的病例。52例显示疣状和基底样类型的各种组合，7例显示混合形式。结果表明，疣状和基底样类型的纯形式可能不存在。通过PCR在51/58例（88%）中检测到HPV DNA（45例为HPV 16型，6例为HPV 33型），均匀分布于所有年龄组和所有病变类型（WHO和Toki等人，1991年）。通过ISH在24/62例（39%）中检测到HPV（21例为HPV 16/18型，3例为HPV 31/33型），几乎总是在疣状区域。所有这些病例通过PCR对同一病毒类型呈阳性。没有病例显示超过一种HPV类型。PCR未检测到HPV 6、11、18和31型。结果表明HPV 16型和33型与外阴发育异常/原位癌之间存在相关性。
