Rømer M U, Christiansen J, Brünner N, Spang-Thomsen M
Institute of Pathological Anatomy, University of Copenhagen, Denmark.
APMIS. 1995 Jul-Aug;103(7-8):582-7. doi: 10.1111/j.1699-0463.1995.tb01409.x.
The small cell lung cancer cell lines GLC-2 and DMS 456 were genetically labeled with the lacZ gene and examined for invasive and metastatic potential in META/Bom nude mice. The lacZ gene encodes the enzyme beta-D- galactosidase, and cells expressing this enzyme were identified by staining with the chromogenic substrate X-gal. lacZ expressing cells were investigated after subcutaneous (s.c.) inoculation and intravenous (i.v.) injection. The X-gal detection of beta-D-galactosidase activity proved to be a rapid and easy means for specific and highly sensitive identification of metastases. All primary s.c. tumors stained by X-gal. The primary tumors of GLC-2 regularly demonstrated local invasive growth and produced multiple metastases in several organs. In contrast, primary DMS 456 tumors only occasionally demonstrated local invasion and very rarely generated secondary foci. No experimental metastases were found after i.v. injection of the examined tumor lines. The results indicate an intratumoral heterogeneity among individual SCLC tumors in the capacity for invasion and metastatic spread. The different metastatic pattern of GLC-2 after s.c. and i.v. inoculation supports the hypothesis that initial steps of the metastatic cascade occurring in the primary tumor are necessary for the subsequent production of growing metastases.
将小细胞肺癌细胞系GLC - 2和DMS 456用lacZ基因进行基因标记，并在META/Bom裸鼠中检测其侵袭和转移潜能。lacZ基因编码β - D - 半乳糖苷酶，通过用显色底物X - gal染色来鉴定表达该酶的细胞。在皮下（s.c.）接种和静脉内（i.v.）注射后研究表达lacZ的细胞。X - gal检测β - D - 半乳糖苷酶活性被证明是一种快速且简便的方法，用于特异性和高度灵敏地鉴定转移灶。所有原发性皮下肿瘤经X - gal染色。GLC - 2的原发性肿瘤经常表现出局部侵袭性生长，并在多个器官产生多处转移。相比之下，原发性DMS 456肿瘤仅偶尔表现出局部侵袭，很少产生继发性病灶。静脉注射所检测的肿瘤细胞系后未发现实验性转移。结果表明，小细胞肺癌个体肿瘤之间在侵袭和转移扩散能力方面存在肿瘤内异质性。GLC - 2在皮下和静脉接种后的不同转移模式支持了这样的假说，即原发性肿瘤中发生的转移级联反应的初始步骤对于随后生长性转移灶的产生是必要的。
