Didier R, Dastugue B, Meiniel A
Laboratoire de Biochimie Médicale, INSERM U.384, Université d'Auvergne, Clermont-Ferrand, Francec.
Int J Dev Biol. 1995 Jun;39(3):493-9.
The subcommissural organ (SCO) is a cerebral gland that releases into the cerebrospinal fluid a carbohydrate-rich glycoprotein which condenses to form Reissner's fiber (RF). Western blots from two-dimensional gel electrophoresis were stained with lectins (Concanavalin-A, wheat germ agglutinin) and anti-bovine RF serum to identify the secretory products of the chick embryo SCO. Immunohistochemical investigations showed that the anti-bovine RF serum reacted exclusively with the secretion of the SCO. Comparative protein patterns of SCO, pineal organ and cerebral hemisphere extracts allowed us to characterize a specific polypeptide in the SCO electrophoretic profiles. The polypeptide was a highly acid compound (isoelectric point of 4.7) with a high molecular weight (390 kDa). On Western blots only this component was immunoreactive with the RF antiserum and it exhibited an affinity for the two lectins. On the basis of these results, this polypeptide may be considered as a specific component of the secretory material synthesized by the SCO cells of the chick embryo.
连合下器官（SCO）是一种脑腺，它向脑脊液中释放一种富含碳水化合物的糖蛋白，这种糖蛋白会凝聚形成赖氏纤维（RF）。对二维凝胶电泳的蛋白质免疫印迹用凝集素（刀豆球蛋白A、麦胚凝集素）和抗牛RF血清进行染色，以鉴定鸡胚SCO的分泌产物。免疫组织化学研究表明，抗牛RF血清仅与SCO的分泌物发生反应。SCO、松果体器官和大脑半球提取物的比较蛋白质图谱使我们能够在SCO电泳图谱中鉴定出一种特定的多肽。该多肽是一种高分子量（390 kDa）的高酸性化合物（等电点为4.7）。在蛋白质免疫印迹上，只有这种成分与RF抗血清发生免疫反应，并且它对这两种凝集素表现出亲和力。基于这些结果，这种多肽可被认为是鸡胚SCO细胞合成的分泌物质的一种特定成分。
