聚乙二醇修饰的抗生物素蛋白：一种用于在双水相系统中选择性提取生物素化免疫复合物的新型试剂。

Polyethylene glycol-modified avidin: a novel agent for the selective extraction of biotinylated immune-complex in an aqueous two-phase system.

作者信息

Nishimura H, Munakata N, Hayashi K, Hayakawa M, Iwamoto H, Terayama S, Takahata Y, Kodera Y, Tsurui H, Shirai T

机构信息

Department of Material Science and Technology, Toin University of Yokohama, Japan.

出版信息

J Biomater Sci Polym Ed. 1995;7(3):289-96. doi: 10.1163/156856295x00328.

DOI:10.1163/156856295x00328

PMID:7577831

Abstract

Chicken avidin was chemically modified with 2,4-bis[O-methoxypoly(ethylene glycol)]-6-chloro-s-triazine (activated PEG2) to form PEG-avidin. The PEG-avidin, in which 78% of the amino groups were modified, retained 49% of the active biotin-binding sites. The modified avidin was partitioned preferentially into the PEG-phase in an aqueous two-phase system (PEG/dextran). Using PEG-avidin, the immune-complex formed between biotinylated anti-mouse IgG and its antigen IgG (mouse) molecules, was successfully transferred into the PEG-phase in an aqueous two-phase system. This finding leads to the effective isolation of a specific antigen among various kinds of antigens by partitioning with a two-phase system using PEG-avidin.

摘要

用2,4-双[O-甲氧基聚（乙二醇）]-6-氯-s-三嗪（活化的PEG2）对鸡抗生物素蛋白进行化学修饰以形成聚乙二醇化抗生物素蛋白（PEG-抗生物素蛋白）。在PEG-抗生物素蛋白中，78%的氨基被修饰，保留了49%的活性生物素结合位点。在水相双相系统（聚乙二醇/葡聚糖）中，修饰后的抗生物素蛋白优先分配到聚乙二醇相中。使用PEG-抗生物素蛋白，生物素化的抗小鼠IgG与其抗原IgG（小鼠）分子之间形成的免疫复合物成功地转移到水相双相系统的聚乙二醇相中。这一发现导致通过使用PEG-抗生物素蛋白的双相系统分配，在各种抗原中有效分离特定抗原。