圆二色光谱揭示细菌反应中心中P+的不对称性。
The asymmetry of P+ in bacterial reaction centers revealed by circular dichroism spectroscopy.
作者信息
Olson J M, Miller M, D'Olieslager J
机构信息
Institute of Biochemistry, Odense University, Denmark.
出版信息
Biochemistry. 1995 Nov 21;34(46):15230-4. doi: 10.1021/bi00046a031.
The circular dichroism anisotropy, (AL-AR)/A, has been measured for the far-red absorption band of P+ in reaction centers of two purple bacteria (Rhodopseudomonas viridis and Rhodobacter sphaerides) and one green sulfur bacterium (Chlorobium tepidum). The anisotropy values for P960+ (Rps. virdis) at 1310 nm was found to be +(13 +/- 2) x 10(-4). The corresponding for P870+ (Rb. sphaeroides) at 1250 nm was +(11 +/- 1) x 10(-4), but for P840+ (C. tepdium) at 1160 nm the value was negative: -(27 +/- 2) x 10(-4). These results show that the configuration of the special pair in P840 is significantly different from the configuration in P870 and P960.
已对两种紫色细菌（绿脓红假单胞菌和球形红杆菌）以及一种绿色硫细菌（嗜温绿菌）反应中心中P⁺的远红吸收带的圆二色性各向异性（(AL-AR)/A）进行了测量。在1310nm处，P960⁺（绿脓红假单胞菌）的各向异性值为+(13±2)×10⁻⁴。在1250nm处，P870⁺（球形红杆菌）的相应值为+(11±1)×10⁻⁴，但在1160nm处，P840⁺（嗜温绿菌）的值为负：-(27±2)×10⁻⁴。这些结果表明，P840中特殊对的构型与P870和P960中的构型有显著差异。
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