Even M T, Kassner R J, Dolata M, Meyer T E, Cusanovich M A
Department of Chemistry, University of Illinois at Chicago 60607, USA.
Biochim Biophys Acta. 1995 Oct 10;1231(3):220-2. doi: 10.1016/0005-2728(95)00101-n.
The gene for cytochrome c' from Chromatium vinosum was cloned from a HindIII-SalI digest of genomic DNA. A 1.4 kbp fragment containing the gene was sequenced in both directions using the Sanger dideoxy method. The cytochrome c' gene codes for a 154-residue peptide, of which the last 131 amino acids match the previously determined sequence of the protein. The remaining 23 residues represent a signal sequence that is cleaved from the polypeptide upon translocation to the periplasmic space. An additional open reading frame on the other strand of the fragment codes for a peptide that contains four regions that are homologous to corresponding regions of the cytochrome b-type subunit of several Ni-Fe hydrogenases.
