Roudani S, Miralles F, Margotat A, Escribano M J, Lombardo D
INSERM U-260, Faculté de Médecine-Timone, Marseille, France.
Biochim Biophys Acta. 1995 Oct 17;1264(1):141-50. doi: 10.1016/0167-4781(95)00141-3.
In human fetal pancreas, we identified two cDNA transcripts of the bile salt-dependent lipase (BSDL) using reverse transcription followed by polymerase chain reaction (RT-PCR). The sequence of four overlapping segments obtained by RT-PCR matched the sequence of the 2.2 kb cDNA cloned from human adult pancreas (Reue et al. (1991) J. Lipid Res. 32, 267-276). A second RT-PCR product of approx. 1.1 kb was evidenced, the sequence of which corresponds to that of the BSDL-pseudogene transcript (Nilsson et al. (1993) Genomics, 17, 416-422). The short transcript is present in all tissues examined whereas the former one (2.2 kb) is either poorly (in liver and kidney) or not at all expressed in adult tissues, excepted in the pancreas. On the other hand, the 2.2 kb transcript specific of the BSDL gene was detected in all fetal tissues examined as early as the 6th week of gestation. Results also suggested that the fetal pancreas contains more 2.2 kb transcript than its adult counterpart. Therewith, BSDL was immuno-precipitated from fetal liver. The role of BSDL-gene expression during the fetal life is discussed.
在人胎儿胰腺中，我们采用逆转录随后进行聚合酶链反应（RT-PCR）的方法，鉴定出了胆汁盐依赖性脂肪酶（BSDL）的两种cDNA转录本。通过RT-PCR获得的四个重叠片段的序列，与从成人胰腺克隆的2.2 kb cDNA的序列相匹配（Reue等人，(1991) 《脂质研究杂志》32卷，267 - 276页）。还证实了约1.1 kb的第二种RT-PCR产物，其序列与BSDL假基因转录本的序列相对应（Nilsson等人，(1993) 《基因组学》17卷，416 - 422页）。短转录本存在于所有检测的组织中，而前一种（2.2 kb）在成体组织中表达不佳（在肝脏和肾脏中）或根本不表达，胰腺除外。另一方面，早在妊娠第6周，在所有检测的胎儿组织中就检测到了BSDL基因特有的2.2 kb转录本。结果还表明，胎儿胰腺中2.2 kb转录本的含量比成年胰腺中的多。据此，从胎儿肝脏中免疫沉淀出了BSDL。文中讨论了胎儿期BSDL基因表达的作用。
