Samoĭlenko S G, Okun' I M, Aksentsev S L, Konev S V
Biofizika. 1992 Mar-Apr;37(2):290-4.
Using fluorescent probe pyrene a study was carried out of temperature dependence of the annular and lipid bilayer polarity in synaptic membranes. Polarity of microenvironment was evaluated by a ratio of intensities of vibronic bands in pyrene fluorescence spectra at direct excitation of probe or through energy transfer from protein molecules. At the temperature range 10-50 degrees C the polarity of bilayer was shown to increase while that of annular lipid underwent biphasic changes. Above 20 degrees C polarity of the bilayer was higher than of the annular zone. From experiments on pyrene fluorescence quenching with potassium iodide the conclusion has been made that changes in polarity correlate with pyrene accessibility to water.
使用荧光探针芘对突触膜中环形和脂质双层极性的温度依赖性进行了研究。通过在直接激发探针时或通过从蛋白质分子的能量转移来评估芘荧光光谱中振动带的强度比，从而对微环境的极性进行评估。在10 - 50摄氏度的温度范围内，双层的极性显示出增加，而环形脂质的极性则经历双相变化。在20摄氏度以上，双层的极性高于环形区域。通过碘化钾对芘荧光猝灭的实验得出结论，极性的变化与芘对水的可及性相关。
