Cremer M A, Griffiths M M, Terato K, Kang A H
Research Service, Veterans Administration Medical Center, University of Tennessee, Memphis 38104, USA.
Autoimmunity. 1995;20(3):153-61. doi: 10.3109/08916939508993346.
To determine the relationship between susceptibility to bovine type XI and II (BXI and BII) collagen-induced arthritis, we immunized 14 inbred and one outbred strains of rats with BXI and BII. Susceptibility to BXI-arthritis corresponded largely with susceptibility, or resistance, to BII-arthritis. LEW, BB, WF, DA, and WKY were readily susceptible to BXI- and BII-arthritis. Likewise, BII-resistant F344 and BN rats were BXI-resistant. Some strains responded differently to BXI and BII. BUF and COP, which are moderately susceptible to BII, were BXI-resistant, whereas the BII-resistant rats, DA.1N and WF.1N, were partially susceptible to BXI. (F344 x BN) F1 hybrids responded to both collagens suggesting gene complementation. Arthritis occurred in all strains producing the highest titer antisera (LEW, WF and BB). Antibody responses to BXI and BII were generally commensurate within individual strains. DA were susceptible to arthritis but produced low levels of antibody comparable to BN rats which were arthritis-resistant. BXI and BII-susceptibility was variable in rats producing intermediate antibody responses. Antibodies to RXI were detected in all BXI-immunized rats, whereas antibodies to RV and RII were uniformly weaker. DTH to RXI and RII was strong in both groups of rats, correlating poorly with arthritis and antibody responses. These studies show that phenotypic susceptibility to BXI- and BII-arthritis are largely concordant among inbred rat strains but clear differences exist in certain strains; multiple genes are likely involved.
为了确定大鼠对牛XI型和II型（BXI和BII）胶原诱导性关节炎易感性之间的关系，我们用BXI和BII免疫了14个近交系和1个远交系大鼠。对BXI关节炎的易感性在很大程度上与对BII关节炎的易感性或抗性相对应。LEW、BB、WF、DA和WKY对BXI和BII关节炎均易感性高。同样，对BII有抗性的F344和BN大鼠对BXI也有抗性。一些品系对BXI和BII的反应不同。对BII中度易感的BUF和COP对BXI有抗性，而对BII有抗性的大鼠DA.1N和WF.1N对BXI部分易感。（F344×BN）F1杂种对两种胶原均有反应，提示基因互补。关节炎发生在所有产生最高滴度抗血清的品系（LEW、WF和BB）中。各个品系内对BXI和BII的抗体反应通常是相称的。DA易患关节炎，但产生的抗体水平较低，与抗关节炎的BN大鼠相当。在产生中等抗体反应的大鼠中，BXI和BII易感性是可变的。在所有用BXI免疫的大鼠中均检测到针对RXI的抗体，而针对RV和RII的抗体始终较弱。两组大鼠对RXI和RII的迟发型超敏反应均较强，与关节炎和抗体反应的相关性较差。这些研究表明，近交系大鼠对BXI和BII关节炎的表型易感性在很大程度上是一致的，但某些品系存在明显差异；可能涉及多个基因。
