Scheller N, Sangaiah R, Ranasinghe A, Amarnath V, Gold A, Swenberg J A
Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill 27599, USA.
Chem Res Toxicol. 1995 Apr-May;8(3):333-7. doi: 10.1021/tx00045a003.
Using readily available labeled compounds, [4,5,6,8-(13)C4]guanine was synthesized in high overall yield. Intermediates as well as the final product were characterized by 1H NMR, 13C NMR, and high resolution mass spectrometry. The labeled guanine was used to generate [13C4]-labeled analogs of the guanine adducts, N2,3-ethenoguanine and 7-(2-hydroxyethyl)guanine. The application of such adducts in isotope dilution mass spectrometry was illustrated with DNA samples from rats exposed to two different mutagenic compounds, vinyl chloride and ethylene oxide.
利用容易获得的标记化合物，以高总收率合成了[4,5,6,8-(13)C4]鸟嘌呤。通过1H NMR、13C NMR和高分辨率质谱对中间体以及最终产物进行了表征。用标记的鸟嘌呤制备了鸟嘌呤加合物N2,3-乙烯基鸟嘌呤和7-(2-羟乙基)鸟嘌呤的[13C4]标记类似物。用暴露于两种不同诱变化合物氯乙烯和环氧乙烷的大鼠的DNA样本说明了此类加合物在同位素稀释质谱中的应用。
