Weinstein Y, Askew D S, Miura O, Cleveland J L, Ihle J N
Faculty of Health Sciences, Unit of Microbiology and Immunology, Ben Gurion University of the Negev, Beer Sheva, Israel.
Eur Cytokine Netw. 1995 Mar-Apr;6(2):97-102.
Murine IL-3-dependent myeloid cell lines express transcripts from non-rearranged TCR gamma genes and this expression is dependent upon IL-3. To investigate this observation in general terms we examined various IL-3 dependent cell lines for TCR gamma gene expression. We also examined various cytokines to test their potential to induce TCR gamma gene expression. All IL-3 dependent cell lines expressed TCR gamma transcripts. The IL-3 induced expression was sensitive to protein synthesis inhibitors. This demonstrated that the TCR gamma genes belong to the early growth factor response class. IL-3, IL-4, GM-CSF and Erytropoietin (EPO), but not G-CSF, induced TCR gene expression. 32D cells transfected with the IL-2 beta chain receptor became responsive to IL-2 as a growth factor and induced TCR gamma gene expression. The induction of TCR gamma gene expression by the cytokines was not correlated to their growth promoting activity. This indicated different signaling pathways.
小鼠白细胞介素3（IL-3）依赖的髓样细胞系表达来自未重排的T细胞受体γ（TCRγ）基因的转录本，且这种表达依赖于IL-3。为了从总体上研究这一现象，我们检测了多种IL-3依赖的细胞系的TCRγ基因表达。我们还检测了多种细胞因子，以测试它们诱导TCRγ基因表达的潜力。所有IL-3依赖的细胞系均表达TCRγ转录本。IL-3诱导的表达对蛋白质合成抑制剂敏感。这表明TCRγ基因属于早期生长因子反应类别。IL-3、IL-4、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和促红细胞生成素（EPO），但不包括粒细胞集落刺激因子（G-CSF），可诱导TCR基因表达。转染了白细胞介素2β链受体的32D细胞对作为生长因子的IL-2产生反应并诱导TCRγ基因表达。细胞因子对TCRγ基因表达的诱导与其促生长活性无关。这表明存在不同的信号通路。
