神经营养因子-3与睫状神经营养因子联合应用可促进蜗轴-螺旋神经节外植体中神经元的存活。

NT-3 combined with CNTF promotes survival of neurons in modiolus-spiral ganglion explants.

作者信息

Staecker H, Liu W, Hartnick C, Lefebvre P, Malgrange B, Moonen G, Van de Water T R

机构信息

Department of Otolaryngology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

出版信息

Neuroreport. 1995 Jul 31;6(11):1533-7. doi: 10.1097/00001756-199507310-00017.

DOI:10.1097/00001756-199507310-00017

PMID:7579142

Abstract

Auditory neurons depend upon the integrity of both their peripheral (auditory hair cells) and central (cochlear nucleus) targets for survival. One proposed trophic mechanism is the production of neurotrophin-3 (NT-3) by auditory hair cells. Modiolus-spiral ganglion explants from adult rats that closely mirror cell-cell interactions and in vivo tissue relationships within this ganglion provide a model for testing trophic factors. Brain derived neurotrophic factor (BDNF), NT-3 and ciliary neurotrophic factor (CNTF) were tested for their ability, both individually and in combination, to support neuronal survival. NT-3 was the strongest individual promoter of survival, while CNTF (a cytokine) with NT-3 (a neurotrophin) was the most effective combination for promoting the survival of auditory neurons.

摘要

听觉神经元的存活依赖于其外周（听觉毛细胞）和中枢（耳蜗核）靶点的完整性。一种提出的营养机制是听觉毛细胞产生神经营养因子-3（NT-3）。来自成年大鼠的蜗轴-螺旋神经节外植体紧密反映了该神经节内的细胞间相互作用和体内组织关系，为测试营养因子提供了一个模型。对脑源性神经营养因子（BDNF）、NT-3和睫状神经营养因子（CNTF）单独及联合支持神经元存活的能力进行了测试。NT-3是最强的单个存活促进因子，而CNTF（一种细胞因子）与NT-3（一种神经营养因子）联合是促进听觉神经元存活的最有效组合。