Rusakov D A, Stewart M G, Sojka M, Richter-Levin G, Bliss T V
Department of Biology, The Open University, Milton Keynes, London, UK.
Neuroreport. 1995 Jul 31;6(11):1557-61. doi: 10.1097/00001756-199507310-00023.
A quantitative study of the distribution of dendritic spines was carried out in three orders of dendritic branches of granule cells from the dentate gyrus of the rat hippocampus. Golgi-stained preparations (7-19 neurones in each of seven rats) were analysed using computerized microscopy. Identification of spines and quantification of stem-spine geometry was performed using a segmentation algorithm and a line skeleton transformation of dendritic images. Analysis of data using the statistics of point processes revealed that, in all three branch orders, the distribution of visible spines along dendrites was not evenly random, but included dense clusters of spines surrounding the dendritic stem (spine 'collars'). Three-dimensional reconstructions from serial ultrathin sections have confirmed the presence of such spine groups. We speculate the spine collars represent a functional element in which associative synaptic plasticity is fostered by the proximity of individual synapses.
对大鼠海马齿状回颗粒细胞的三个树突分支等级中的树突棘分布进行了定量研究。使用计算机显微镜分析了高尔基染色制剂（七只大鼠中的每只大鼠有7 - 19个神经元）。使用分割算法和树突图像的线骨架变换对树突棘进行识别并对树突 - 树突棘几何形状进行定量。使用点过程统计对数据进行分析后发现，在所有三个分支等级中，沿树突的可见树突棘分布并非均匀随机，而是包括围绕树突干的密集树突棘簇（树突棘“环”）。来自连续超薄切片的三维重建证实了此类树突棘群的存在。我们推测树突棘环代表一种功能元件，其中单个突触的接近促进了联合突触可塑性。
