Hewett S J, Choi D W, Gutmann D H
Department of Neurology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Neuroreport. 1995 Jul 31;6(11):1565-8. doi: 10.1097/00001756-199507310-00025.
Neurofibromin, the product of the neurofibromatosis 1 (NF1) gene, is an important tumor suppressor protein expressed most abundantly in the nervous system. Within the central nervous system, neurofibromin has been localized to neurons and oligodendrocytes but not astrocytes. As individuals with NF1 are at an increased risk for optic pathway gliomas and astrocytomas, we chose to re-evaluate the level of neurofibromin expression in primary cultures of murine cortical astrocytes under control and reactive conditions. Astrocytes under control conditions expressed low levels of NF1 mRNA and protein. Following stimulation with dibutyryl-cyclic AMP or interferon-gamma in combination with either lipopolysaccharide or interleukin-1 beta, there was a marked increase in NF1 mRNA and protein expression. These results suggest that neurofibromin may be involved in the process of reactive astrocytosis seen in response to CNS injury.
神经纤维瘤蛋白是神经纤维瘤病1（NF1）基因的产物，是一种重要的肿瘤抑制蛋白，在神经系统中表达最为丰富。在中枢神经系统内，神经纤维瘤蛋白已定位到神经元和少突胶质细胞，但未定位到星形胶质细胞。由于NF1患者患视神经通路胶质瘤和星形细胞瘤的风险增加，我们选择在对照和反应性条件下重新评估小鼠皮质星形胶质细胞原代培养物中神经纤维瘤蛋白的表达水平。对照条件下的星形胶质细胞表达低水平的NF1 mRNA和蛋白。在用二丁酰环磷酸腺苷或干扰素-γ联合脂多糖或白细胞介素-1β刺激后，NF1 mRNA和蛋白表达显著增加。这些结果表明，神经纤维瘤蛋白可能参与了中枢神经系统损伤后出现的反应性星形胶质细胞增生过程。
