Trentmann S M, Kende H
MSU-DOE Plant Research Laboratory, East Lansing, MI 48824-1312, USA.
Plant Mol Biol. 1995 Oct;29(1):161-6. doi: 10.1007/BF00019127.
The negative regulatory protein of ethylene synthesis in ripening tomato fruit, E8, is structurally related to the enzyme that catalyzes the last step in ethylene synthesis, 1-aminocyclopropane-1-carboxylate (ACC) oxidase, and to a large family of 2-oxoglutarate-dependent dioxygenases (2-ODD). A cDNA with structural homology to the tomato E8 was isolated from a cDNA library of Arabidopsis thaliana. Sequence analysis showed that this cDNA, 2A6, encodes a protein of 361 amino acids. Southern blot analysis indicated that the corresponding gene is unique in the Arabidopsis genome. The level of the 2A6 transcript was not increased by ethylene in siliques of Arabidopsis, as was E8 in tomato fruits, and was also expressed in etiolated seedlings, leaves, stems and flowers. The 2A6 protein shows three domains that are highly conserved among E8, ACC oxidases, and 2-ODDs.
成熟番茄果实中乙烯合成的负调控蛋白E8，在结构上与催化乙烯合成最后一步的酶1-氨基环丙烷-1-羧酸（ACC）氧化酶以及一大类依赖2-氧代戊二酸的双加氧酶（2-ODD）相关。从拟南芥的cDNA文库中分离出了一个与番茄E8具有结构同源性的cDNA。序列分析表明，这个名为2A6的cDNA编码一个由361个氨基酸组成的蛋白质。Southern杂交分析表明，相应的基因在拟南芥基因组中是唯一的。与番茄果实中的E8不同，拟南芥角果中的2A6转录本水平不会因乙烯而升高，并且在黄化幼苗、叶片、茎和花中也有表达。2A6蛋白显示出在E8、ACC氧化酶和2-ODD中高度保守的三个结构域。
