来自类溶血巴斯德氏菌的白细胞毒素分泌决定因素的序列分析。
Sequence analysis of leukotoxin secretion determinants from a Pasteurella haemolytica-like organism.
作者信息
Chang Y F, Ma D P, Wang Y, Chin N, Chengappa M M, Shin S J, Lein D H, Yoon H J
机构信息
Diagnostic Laboratory, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
出版信息
DNA Seq. 1995;5(5):291-7. doi: 10.3109/10425179509030983.
The pHLBD genes encoding the secretion functions for the 105 kDa RTX leukotoxin of Pasteurella haemolytica-like (PHL) organism has been cloned and sequenced. Like analogous genes from other RTX determinants, the pHLBD genes lie immediately downstream from the leukotoxin structural gene, pHLA. Although isolated from a diverse group of gram-negative organisms, the pHLBD genes and the characterized RTX BD genes from other organisms exhibit a high degree of homology at both the DNA and predicted amino acid sequence levels. We have previously reported the cloning of the leukotoxin gene (pHLCA) (Chang et al., Infect. Immun. 61:2089-2095), which encodes a 105-kda polypeptide with cytotoxic activity. DNA sequence analysis of the pHLBD genes shows 83.93% and 86.05% homologous to that of P. haemolytica IktBD genes, respectively.
编码类溶血巴斯德氏菌（PHL）105 kDa RTX白细胞毒素分泌功能的pHLBD基因已被克隆和测序。与来自其他RTX决定簇的类似基因一样，pHLBD基因位于白细胞毒素结构基因pHLA的紧邻下游。尽管从多种革兰氏阴性菌中分离得到，但pHLBD基因与其他生物中已鉴定的RTX BD基因在DNA和预测的氨基酸序列水平上都表现出高度同源性。我们之前已报道了白细胞毒素基因（pHLCA）的克隆（Chang等人，《感染与免疫》61:2089 - 2095），该基因编码具有细胞毒性活性的105 kDa多肽。pHLBD基因的DNA序列分析显示，其与溶血巴斯德氏菌IktBD基因的同源性分别为83.93%和86.05%。
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