Wallace C J
Dalhousie University, Halifax, Canada.
Curr Opin Biotechnol. 1995 Aug;6(4):403-10. doi: 10.1016/0958-1669(95)80069-7.
Semisynthesis is used to create defined analogues of proteins by the chemical manipulation of peptide fragments largely derived from the natural protein and the subsequent reassembly of those fragments into a near-native conformation. In common with the total synthesis of proteins, it requires efficient and non-destructive methods for peptide religation. Recently, a wide range of chemoselective ligation schemes have been elaborated that now permit the assembly of minimally protected peptides from either synthetic or natural sources.
半合成用于通过对主要源自天然蛋白质的肽片段进行化学操作，然后将这些片段重新组装成接近天然的构象，来创建特定的蛋白质类似物。与蛋白质的全合成一样，它需要高效且无损的肽连接方法。最近，已经开发出了多种化学选择性连接方案，这些方案现在允许从合成或天然来源组装最低限度保护的肽。
