Chemical techniques employed for the primary structural analysis of proteins and peptides.
作者信息
Inglis A S, Reid G E, Simpson R J
机构信息
Joint Protein Structure Laboratory, Ludwig Institute for Cancer Research (Melbourne Branch) Parkville, Victoria Australia.
出版信息
EXS. 1995;73:141-71. doi: 10.1007/978-3-0348-9061-8_8.
PMID:7579972
Abstract
This chapter summarises modern microchemical approaches to the purification, identification and primary structure analysis of peptides and proteins. Discussion of high-sensitivity purification methods is restricted to two-dimensional polyacrylamide gel electrophoresis (2-DE) and microbore/capillary column reversed-phase high-performance liquid chromatography (RP-HPLC). Associated techniques are discussed, particularly with respect to analysis of the products with current automated amino acid sequencers and mass spectrometers.
摘要
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