使用卤乙酸脱卤酶基因作为大肠杆菌和假单胞菌载体的选择标记。
Use of haloacetate dehalogenase genes as selection markers for Escherichia coli and Pseudomonas vectors.
作者信息
Kawasaki H, Kuriyama H, Tonomura K
机构信息
Department of Agricultural Chemistry, University of Osaka Prefecture, Japan.
出版信息
Biodegradation. 1995 Sep;6(3):213-6. doi: 10.1007/BF00700459.
The haloacetate dehalogenase gene, dehH2, cloned from Moraxella sp. strain B could be used a selection marker gene for vectors in Escherichia coli and Pseudomonas putida. Haloacetates, especially iodoacetate, inhibit the growth of some microorganisms. The dehH2 gene introduced into the cells conferred iodoacetate resistance on them. Therefore, E. coli and P. putida transformed with vectors marked with dehH2 could be easily selected on plates containing iodoacetate.
从莫拉克斯氏菌属菌株B中克隆得到的卤乙酸脱卤酶基因dehH2，可作为大肠杆菌和恶臭假单胞菌载体的选择标记基因。卤乙酸，尤其是碘乙酸，会抑制某些微生物的生长。导入细胞的dehH2基因赋予它们碘乙酸抗性。因此，用标记有dehH2的载体转化的大肠杆菌和恶臭假单胞菌可以很容易地在含有碘乙酸的平板上筛选出来。
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