鲶鱼中嗪草酮、莠去津和扑灭通残留量的液相色谱测定法
Liquid chromatographic determination of simazine, atrazine, and propazine residues in catfish.
作者信息
Holland D C, Munns R K, Roybal J E, Hurlbut J A, Long A R
机构信息
U.S. Food and Drug Administration, Denver Federal Center, CO 80225-0087, USA.
出版信息
J AOAC Int. 1995 Jul-Aug;78(4):1067-71.
A liquid chromatographic (LC) method is described for the simultaneous determination of the triazine herbicides, simazine (SIM), atrazine (ATZ), and propazine (PRO) in the 12.5-100 ppb range in catfish. The herbicides are extracted from catfish homogenates with ethyl acetate, followed by solvent partitioning between acetonitrile and petroleum ether and additional cleanup on a C18 cartridge. A Supelcosil LC-18-DB column is used for LC separation, and UV determination is at 220 nm. The isocratic mobile phase is a mixture of methanol, acetonitrile, and water. Mean recoveries from catfish were 88.7, 96.9, and 91.7%; standard deviations were 6.84, 7.78, and 6.26%; and coefficients of variation were 7.72, 8.03, and 6.82% for SIM, ATZ, and PRO, respectively.
描述了一种液相色谱（LC）方法，用于同时测定鲶鱼中浓度范围为12.5 - 100 ppb的三嗪类除草剂西玛津（SIM）、莠去津（ATZ）和扑灭通（PRO）。用乙酸乙酯从鲶鱼匀浆中提取除草剂，然后在乙腈和石油醚之间进行溶剂分配，并在C18柱上进一步净化。使用Supelcosil LC - 18 - DB柱进行LC分离，在220 nm处进行紫外测定。等度流动相为甲醇、乙腈和水的混合物。鲶鱼中的平均回收率分别为88.7%、96.9%和91.7%；标准偏差分别为6.84%、7.78%和6.26%；SIM、ATZ和PRO的变异系数分别为7.72%、8.03%和6.82%。
Zotero 插件