Di Corcia A, Marchetti M, Samperi R
Dipartimento di Chimica, Università La Sapienza di Roma, Italy.
J Chromatogr. 1987 Sep 18;405:357-63. doi: 10.1016/s0021-9673(01)81777-5.
A rapid and simple method for the determination of trace amounts of simazine and atrazine in water is described. A 250-ml volume of water is pre-concentrated by passage at a flow-rate of about 30 ml/min through a small trap containing 50 mg of graphitized carbon black (Carbopack B). After washing with 150 microliters of methanol, the two herbicides are desorbed with 700 microliters of dichloromethane-methanol (60:40, v/v). After removal, of the solvent, the extracted sample is fractionated and analysed by reversed-phase high-performance liquid chromatography with UV detection at 220 nm. A single assay can be completed within 40 min from the receipt of the water sample. Recoveries of simazine and atrazine added to 250 ml of water at the level of 50 ng/l were 97.2 and 95.8% and the limits of detection were 0.07 and 0.15 ng. respectively. At the 50 ng/l level in water, the coefficients of variation for simazine and atrazine were 3.7 and 4.0% (n = 7), respectively.
本文描述了一种快速简便的测定水中痕量西玛津和莠去津的方法。取250毫升水样，以约30毫升/分钟的流速通过装有50毫克石墨化炭黑（Carbopack B）的小型捕集阱进行预浓缩。用150微升甲醇洗涤后，用700微升二氯甲烷 - 甲醇（60:40，v/v）解吸两种除草剂。去除溶剂后，将萃取的样品进行分馏，并通过反相高效液相色谱法在220纳米处进行紫外检测分析。从收到水样起，40分钟内即可完成一次测定。向250毫升水中添加50纳克/升水平的西玛津和莠去津，回收率分别为97.2%和95.8%，检测限分别为0.07和0.15纳克。在水中50纳克/升的水平下，西玛津和莠去津的变异系数分别为3.7%和4.0%（n = 7）。
