Harik-Khan R, Moats W A
U.S. Department of Agriculture, Meat Science Research Laboratory, Beltsville, MD 20705, USA.
J AOAC Int. 1995 Jul-Aug;78(4):978-86.
A procedure for identifying and quantitating violative beta-lactams in milk is described. This procedure integrates beta-lactam residue detection kits with the multiresidue automated liquid chromatographic (LC) cleanup method developed in our laboratory. Spiked milk was deproteinized, extracted, and subjected to reversed-phase LC using a gradient program that concentrated the beta-lactams. Amoxicillin, ampicillin, cephapirin, ceftiofur, cloxacillin, and penicillin G were, thus, separated into 5 fractions that were subsequently tested for activity by using 4 kits. beta-lactams in the positive fractions were quantitated by analytical LC methods developed in our laboratory. The LC cleanup method separated beta-lactam antibiotics from each other and from interferences in the matrix and also concentrated the antibiotics, thus increasing the sensitivity of the kits to the beta-lactam antibiotics. The procedure facilitated the task of identifying and measuring the beta-lactam antibiotics that may be present in milk samples.
本文描述了一种用于鉴定和定量牛奶中违规β-内酰胺类药物的方法。该方法将β-内酰胺残留检测试剂盒与我们实验室开发的多残留自动液相色谱（LC）净化方法相结合。向加标牛奶中加入蛋白质沉淀剂进行去蛋白处理，然后进行提取，并使用梯度程序进行反相LC分析，以浓缩β-内酰胺类药物。阿莫西林、氨苄西林、头孢匹林、头孢噻呋、氯唑西林和青霉素G因此被分离成5个组分，随后使用4种试剂盒对其活性进行检测。通过我们实验室开发的分析LC方法对阳性组分中的β-内酰胺类药物进行定量。LC净化方法将β-内酰胺类抗生素彼此分离，并与基质中的干扰物分离，同时还浓缩了抗生素，从而提高了试剂盒对β-内酰胺类抗生素的灵敏度。该方法有助于鉴定和测量牛奶样品中可能存在的β-内酰胺类抗生素。
