使用福林-西奥尔特苯酚反应测定磷酸酪氨酸磷酸酶活性。
Measurement of phosphotyrosine phosphatase activity using the Folin-Ciocalteu phenol reaction.
作者信息
Gmeiner B M, Seelos C C
机构信息
Institute of Medical Chemistry, University of Vienna, Austria.
出版信息
Biochem Mol Biol Int. 1995 Aug;36(5):943-8.
PMID:7581010
Abstract
An assay for the estimation of phosphotyrosine phosphatase using the Folin-Ciocalteu phenol reaction to monitor enzyme activity is presented. The method is based on the property of the substrate phosphotyrosine not to react as a phenol until it is dephosphorylated. The method is sensitive, there is no interference from the use of detergents and it does not rely on special laboratory equipment to distinguish tyrosine from phosphotyrosine.
摘要
本文介绍了一种利用福林-西奥尔特酚反应监测酶活性来估算磷酸酪氨酸磷酸酶的分析方法。该方法基于底物磷酸酪氨酸在去磷酸化之前不会像酚一样发生反应的特性。该方法灵敏，使用洗涤剂不会产生干扰，并且不依赖特殊实验室设备来区分酪氨酸和磷酸酪氨酸。
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