Kumar S, Suzuki H, Onoue S, Suzuki S, Hattori N, Ozawa T
Department of Biomedical Chemistry, Faculty of Medicine, University of Nagoya, Japan.
Biochem Mol Biol Int. 1995 Aug;36(5):973-81.
We describe two new inter-specifically conserved sequences named Mt5 and Mt6 elements which are around the termination associated sequence (TAS) at the 3' end of the displacement loop (D-loop) region of vertebrate mtDNAs. We employed gel mobility shift assay to search for D-loop-binding proteins using partially purified rat mitochondrial mtDNA-binding proteins and 10 human DNA fragments covering the entire D-loop region as probe DNAs. Competition binding assays indicated the presence of four different rat mitochondrial D-loop-binding proteins. Each of the rat proteins bound, in a sequence-specific manner, to a probe DNA containing an inter-specifically conserved sequence; TAS, Mt5, B block, and a A+T-rich sequence overlapping the conserved sequence block I.
我们描述了两个新的种间保守序列，命名为Mt5和Mt6元件，它们位于脊椎动物线粒体DNA位移环（D-loop）区域3'端的终止相关序列（TAS）附近。我们采用凝胶迁移率变动分析，使用部分纯化的大鼠线粒体mtDNA结合蛋白和覆盖整个D-loop区域的10个人类DNA片段作为探针DNA，来寻找D-loop结合蛋白。竞争结合分析表明存在四种不同的大鼠线粒体D-loop结合蛋白。每种大鼠蛋白都以序列特异性方式与包含种间保守序列的探针DNA结合；TAS、Mt5、B区以及与保守序列块I重叠的富含A+T的序列。
