软骨藻酸在胚胎期鸡视网膜的培养细胞中诱导神经毒性和肌醇三磷酸动员。

Domoic acid induces neurotoxicity and ip3 mobilization in cultured cells of embryonic chick retina.

作者信息

Duran R, Reis R A, Almeida O M, de-Mello M C, de-Mello F G

机构信息

Laboratório de Neuroquímica, Universidade Federal do Rio de Janeiro, Brasil.

出版信息

Braz J Med Biol Res. 1995 Jan;28(1):100-7.

PMID:7581017

Abstract

Domoic acid (DOM), 1 to 50 microM, a glutamate agonist responsible for several neurological effects such as loss of memory and confusion, induced the death of cultured neurons of chick embryonic retina, in a concentration- and Ca(2+)-dependent manner. This effect was blocked by 100 microM CNQX, a competitive antagonist of the non-NMDA receptor, but not by 10 microM MK-801, a non-competitive antagonist of the NMDA receptor. DOM also induced inositol triphosphate (ip3) accumulation 4 to 7 times above basal levels. This effect was also dependent on external Ca2+ and was entirely blocked by 100 microM CNQX, but not by 10 microM MK-801. These results suggest that DOM interaction with non-NMDA glutamate receptors mediates signal transduction with ip3 accumulation and cell death.

摘要

软骨藻酸（DOM），浓度为1至50微摩尔，是一种谷氨酸激动剂，会引发多种神经效应，如记忆丧失和意识混乱，它以浓度和钙离子（Ca(2+)）依赖的方式诱导鸡胚视网膜培养神经元死亡。这种效应可被100微摩尔的CNQX（一种非NMDA受体的竞争性拮抗剂）阻断，但不能被10微摩尔的MK-801（一种NMDA受体的非竞争性拮抗剂）阻断。DOM还诱导肌醇三磷酸（IP3）积累至基础水平以上4至7倍。这种效应也依赖于细胞外钙离子，并且完全被100微摩尔的CNQX阻断，但不能被10微摩尔的MK-801阻断。这些结果表明，DOM与非NMDA谷氨酸受体的相互作用通过IP3积累和细胞死亡介导信号转导。