Arruda M S, Montenegro M R
Departamento de Ciências Biológicas, Faculdade de Ciências, Universidade Estadual Paulista, Brasil.
Braz J Med Biol Res. 1995 Feb;28(2):209-12.
We have studied the role of Paracoccidioides brasiliensis viability in the morphology of paracoccidioidomycotic granulomas in the hamster cheek pouch, an immunologically privileged site. Naive (N = 75) and previously sensitized (N = 50) two-month old male hamsters (Mesocricetus auratus) were inoculated into the pouch with 5 x 10(5) live or heat- or formalin-killed fungi. Previously sensitized animals presented a positive footpad test and immunodiffusion demonstrable antibodies (titer 1/32), at the time of sacrifice; naive animals were always negative for those immunological tests. The histological results showed that, like viable P. brasiliensis, killed fungi evoke typical epithelioid granulomas in 100% of animals, even in the absence of immunodiffusion or footpad test detectable immune response. The granulomas elicited by killed fungi were devoid of giant cells or a mononuclear cell halo, suggesting that live proliferating fungi or their products may be involved in these events.
我们研究了巴西副球孢子菌的活力在仓鼠颊囊（一个免疫特惠部位）中副球孢子菌病肉芽肿形态形成中的作用。将5×10⁵个活的、热灭活或甲醛灭活的真菌接种到75只未致敏和50只预先致敏的两个月大雄性仓鼠（金黄仓鼠）的颊囊中。在处死时，预先致敏的动物足垫试验呈阳性，免疫扩散可检测到抗体（效价1/32）；未致敏动物的这些免疫试验始终为阴性。组织学结果表明，与活的巴西副球孢子菌一样，灭活真菌在100%的动物中诱发典型的上皮样肉芽肿，即使在没有免疫扩散或足垫试验可检测到的免疫反应的情况下也是如此。由灭活真菌引起的肉芽肿没有巨细胞或单核细胞晕，这表明活的增殖真菌或其产物可能参与了这些过程。
