克氏锥虫、布氏锥虫和硕大利什曼原虫线粒体和细胞质热休克蛋白70的分子比较。

Molecular comparison of the mitochondrial and cytoplasmic hsp70 of Trypanosoma cruzi, Trypanosoma brucei and Leishmania major.

作者信息

Klein K G, Olson C L, Donelson J E, Engman D M

机构信息

Department of Microbiology-Immunology, Northwestern University Medical School, Chicago 60611, USA.

出版信息

J Eukaryot Microbiol. 1995 Sep-Oct;42(5):473-6. doi: 10.1111/j.1550-7408.1995.tb05893.x.

DOI:10.1111/j.1550-7408.1995.tb05893.x

PMID:7581323

Abstract

We compared the expression and localization of the mitochondrial and cytoplasmic hsp70 of the protozoans Trypanosoma cruzi, Trypanosoma brucei and Leishmania major. The mitochondrial protein is encoded by multiple mRNA in all species, while the cytoplasmic protein is encoded by a single mRNA. In all three species, the mitochondrial hsp70 is concentrated in the kinetoplast, a submitochondrial structure that houses the unusual DNA (kDNA) that characterizes this group of organisms, while the cytoplasmic protein is distributed throughout the cell. These results suggest that, in all kinetoplastid species, mt-hsp70 has a specific function in kDNA biology, possibly in the processes of kDNA replication, RNA editing or kinetoplast structure.

摘要

我们比较了原生动物克氏锥虫、布氏锥虫和硕大利什曼原虫线粒体和细胞质热休克蛋白70（hsp70）的表达及定位情况。在所有物种中，线粒体蛋白由多个mRNA编码，而细胞质蛋白由单个mRNA编码。在这三个物种中，线粒体hsp70都集中在线粒体动粒中，线粒体动粒是一种亚线粒体结构，其中容纳着该类生物体特有的异常DNA（动粒DNA，kDNA），而细胞质蛋白则分布于整个细胞。这些结果表明，在所有动基体目物种中，线粒体hsp70在动粒DNA生物学中具有特定功能，可能参与动粒DNA复制、RNA编辑或线粒体动粒结构形成过程。