Hsu L C, Constable D J, Orvos D R, Hannah R E
Environmental Research Laboratory, SmithKline Beecham Corporation, King of Prussia, PA 19406, USA.
J Chromatogr B Biomed Appl. 1995 Jul 7;669(1):85-92. doi: 10.1016/0378-4347(95)00022-b.
High-performance liquid chromatography (HPLC) and capillary zone electrophoresis (CZE) were used in biodegradation kinetic studies. This paper describes a rapid penciclovir separation using CZE with detection limits comparable to HPLC. The ionic-strength mediated stacking technique was employed while good resolution was maintained. With a shorter analysis time, comparable detection limits and no organic solvent consumption, CZE is a better method for penciclovir biodegradation studies than conventional reversed-phase HPLC (RP-HPLC).
高效液相色谱法（HPLC）和毛细管区带电泳法（CZE）被用于生物降解动力学研究。本文描述了一种使用CZE快速分离喷昔洛韦的方法，其检测限与HPLC相当。采用了离子强度介导堆积技术，同时保持了良好的分离度。与传统的反相高效液相色谱法（RP-HPLC）相比，CZE分析时间更短、检测限相当且无需消耗有机溶剂，是用于喷昔洛韦生物降解研究的更好方法。
