Daley M D, Siminovitch K A
Department of Anesthesia, Allen Memorial Hospital, Waterloo, IA, USA.
New Horiz. 1995 May;3(2):146-60.
Over the past 15 years, knowledge of the molecular pathophysiology of human disease has increased at a remarkable pace. Most of this progress can be attributed to concomitant advances in basic molecular biology and, specifically, the development of an ever-expanding aramentarium of technologies for analysis of gene structure and function referred to as recombinant DNA technology. In this article, we provide an introduction to this technology by describing the basic language, principles, and tools of molecular biology as well as a few of the most widely used recombinant DNA techniques. By necessity, this discussion does not encompass the huge body of available molecular methodologies, but the information provided here should provide a sufficient framework for appreciating how recombinant DNA has already impacted, and how it will most certainly reshape, the practice of medicine.
在过去的15年里，人类疾病分子病理生理学的知识以惊人的速度增长。这一进展大部分可归因于基础分子生物学的同步进步，特别是用于分析基因结构和功能的技术手段不断扩充，即所谓的重组DNA技术。在本文中，我们将通过描述分子生物学的基本语言、原理和工具以及一些最广泛使用的重组DNA技术来介绍这项技术。由于篇幅所限，本讨论并未涵盖所有可用的分子方法，但此处提供的信息应能为理解重组DNA如何已经影响以及必将如何重塑医学实践提供一个充分的框架。
