Papp A, Klee M R
Department of Neurophysiology, Max-Planck-Institute for Brain Research, Frankfurt/M. Germany.
Brain Res. 1995 Jun 26;684(1):107-11. doi: 10.1016/0006-8993(95)00437-u.
Alterations in the parameters of action potentials upon changes in protein kinase C (PKC) activity were studied on neurons of the visceral ganglion of Aplysia californica. The amplitude and maximum speed of the up-and downstroke of the action potentials (APs) were measured. Intracellularly injected PKC and intra- and extracellularly applied phorbol-12,13-diacetate (PDAc) had similar effects on the Aplysia neurons, the most prominent being an increase of the upstroke speed of the AP in every neuron. The non-PKC-activating 4 alpha-phorbol didecanoate had no effect, and the effects of the PKC blocker H-7 were opposite to those of PDAc. It was concluded that the changes of the AP evoked by PDAc are mediated through PKC activation.
在加州海兔内脏神经节的神经元上，研究了蛋白激酶C（PKC）活性变化时动作电位参数的改变。测量了动作电位（AP）上升和下降冲程的幅度和最大速度。细胞内注射PKC以及细胞内和细胞外应用佛波醇-12,13-二乙酸酯（PDAc）对海兔神经元有相似的作用，其中最显著的是每个神经元动作电位上升速度的增加。非PKC激活剂4α-佛波醇二癸酸酯没有作用，PKC阻滞剂H-7的作用与PDAc相反。得出的结论是，PDAc诱发的动作电位变化是通过PKC激活介导的。
