Matsuda H, Akechi Y, Shimada Y, Terasawa K, Watanabe H
Department of Japanese Oriental, Kampo, Medicine, Faculty of Medicine, Japan.
Brain Res. 1995 Jul 17;686(1):111-4. doi: 10.1016/0006-8993(95)00500-p.
In order to discriminate well-lesioned rats after unilateral microinjection of 6-OHDA into the nigrostriatal dopamine system, we measured the spontaneous rotation in the night period and calculated the rate of ipsilateral rotation movement. The rate of ipsilateral rotation movement increased along with the total rotation movement. The rats with over 95% of ipsilateral rotation kept the rate relatively constant for 4 weeks after 6-OHDA lesion and showed a significant increase of contralateral rotation (253.2 +/- 37.9) as compared with the rat that had lower than 95% ipsilateral rotation (3.6 +/- 1.4) after the injection of apomorphine (0.25 mg/kg, s.c.). The reduction percentages of striatal DA contents in animals with unilateral rotation over 95% and under 95% to the lesioned side were 97.8 +/- 0.6% and 59.6 +/- 5.8% (P < 0.001), respectively. The rats with over 90% reduction of striatal DA levels corresponded nicely to rats with 95% ipsilateral rotation among rats injected with apomorphine. These results suggested that the evaluation of ipsilateral rotation, taken the level of 95% rotation to the lesioned side as a standard, was able to discriminate well-lesioned rats without apomorphine treatment after unilateral nigrostriatal 6-OHDA application.
为了鉴别单侧向黑质纹状体多巴胺系统微量注射6-羟基多巴胺（6-OHDA）后损伤良好的大鼠，我们测量了夜间的自发旋转情况，并计算了同侧旋转运动的速率。同侧旋转运动的速率随着总旋转运动而增加。同侧旋转超过95%的大鼠在6-OHDA损伤后4周内保持该速率相对恒定，并且在注射阿扑吗啡（0.25mg/kg，皮下注射）后，与同侧旋转低于95%的大鼠（3.6±1.4）相比，对侧旋转显著增加（253.2±37.9）。单侧旋转超过95%和低于95%的动物纹状体多巴胺含量相对于损伤侧的降低百分比分别为97.8±0.6%和59.6±5.8%（P<0.001）。在注射阿扑吗啡的大鼠中，纹状体多巴胺水平降低超过90%的大鼠与同侧旋转95%的大鼠非常吻合。这些结果表明，以向损伤侧旋转95%为标准评估同侧旋转，能够鉴别单侧黑质纹状体应用6-OHDA后未进行阿扑吗啡处理的损伤良好的大鼠。
