Tassinari P, Deibis L, Blanca I, Bianco N E, Echeverría de Pérez G
Instituto de Inmunología, Facultad de Medicina, Universidad Central de Venezuela, Caracas.
Clin Diagn Lab Immunol. 1995 Jul;2(4):404-7. doi: 10.1128/cdli.2.4.404-407.1995.
To evaluate CD4+/CD29+ cells and their responses to different antigens in polar stages of human immunodeficiency virus (HIV) infection, we studied 26 HIV-seropositive carriers (SPCs) and 15 patients with AIDS simultaneously with 20 healthy volunteers (HVs) and 10 seronegative homosexual and bisexual men (SNH). CD3, CD4, CD29, and CD45RA phenotypes were analyzed by two-color flow cytometry. Significant depletion of CD4+ T cells and both memory (CD4+/CD29+) and naive (CD4+/CD45RA+) T-cell subsets was found among SPCs and AIDS patients compared with the numbers of such cells in the HV and SNH groups. Responses to optimal doses of Candida albicans, streptokinase, and tetanus toxoid were explored in peripheral blood mononuclear cells and CD4(+)- and CD4+/CD29(+)-enriched cell populations. In SPCs, the response to C. albicans in peripheral blood mononuclear cells showed a statistically significant diminution compared with the response of HVs (15,308 versus 35,951 cpm). In addition, a significantly reduced response to streptokinase was evident only when cell preparations were CD4+/CD29+ enriched (3,048 versus 10,367 cpm). Furthermore, the SPC group comprised seven responders to at least one antigen and seven nonresponders to any of the selected specific antigens. Absence of a response in these latter patients was independent of the absolute counts of memory and naive T-cell populations. The response to tetanus toxoid, although diminished in SPCs, was not significantly different from that in controls. Our results suggest that defective responses to common environmental antigens, unrelated to the absolute number of CD4+/CD29+ cells, is probably an early indicator of an HIV-induced lymphocyte lesion.
为了评估人类免疫缺陷病毒（HIV）感染极期CD4⁺/CD29⁺细胞及其对不同抗原的反应，我们同时研究了26名HIV血清反应阳性携带者（SPC）、15名艾滋病患者，以及20名健康志愿者（HV）和10名血清反应阴性的同性恋和双性恋男性（SNH）。通过双色流式细胞术分析CD3、CD4、CD29和CD45RA表型。与HV组和SNH组的此类细胞数量相比，在SPC和艾滋病患者中发现CD4⁺T细胞以及记忆性（CD4⁺/CD29⁺）和初始（CD4⁺/CD45RA⁺）T细胞亚群显著减少。在外周血单核细胞以及富含CD4⁺和CD4⁺/CD29⁺的细胞群体中，探索了对白色念珠菌、链激酶和破伤风类毒素最佳剂量的反应。在SPC中，外周血单核细胞对白色念珠菌的反应与HV相比有统计学意义的降低（15,308对35,951 cpm）。此外，仅当细胞制剂富含CD4⁺/CD29⁺时，对链激酶的反应才明显降低（3,048对10,367 cpm）。此外，SPC组包括7名对至少一种抗原反应者和7名对任何所选特异性抗原无反应者。后一组患者无反应与记忆性和初始T细胞群体的绝对计数无关。尽管SPC对破伤风类毒素的反应有所减弱，但与对照组相比无显著差异。我们的结果表明，对常见环境抗原的反应缺陷，与CD4⁺/CD29⁺细胞的绝对数量无关，可能是HIV诱导淋巴细胞损伤的早期指标。
