Johnson R, Bulfield G, Tait A, Goddard C
Wellcome Unit of Molecular Parasitology, University of Glasgow, U.K.
Comp Biochem Physiol B Biochem Mol Biol. 1995 Mar;110(3):531-7. doi: 10.1016/0305-0491(94)00182-t.
Polyamines are essential requirements for cell proliferation and their role in stimulation of RNA, DNA, and protein synthesis is clearly established. Ornithine decarboxylase is a key enzyme in the biosynthesis of the polyamines and its activity is regulated in response to factors that stimulate cell proliferation. Steady-state ODC mRNA levels and enzyme activity were measured in muscle of chicks genetically selected for increased growth rate or for egg production. In muscle, muscle satellite cells and myotubes, two ODC mRNA transcripts are present of molecular size 2.05 and 1.75 kb. Northern blotting analysis suggest that these transcripts are produced as a result of using different polyadenylation sites. Between day 1 and day 6 after hatching, a period of rapid muscle growth in these animals, a peak in muscle ODC mRNA levels is followed by a peak in enzyme activity in both lines. Significantly higher ODC mRNA levels and enzyme activity are associated with selection for rapid growth in the broiler line. The results are consistent with other data showing that ODC is a major factor in cell growth and provide further evidence that it is a candidate 'trait-gene' for growth.
多胺是细胞增殖的必需物质，其在刺激RNA、DNA和蛋白质合成中的作用已得到明确证实。鸟氨酸脱羧酶是多胺生物合成中的关键酶，其活性受刺激细胞增殖的因子调控。对通过基因选择提高生长速度或产蛋量的雏鸡肌肉中鸟氨酸脱羧酶（ODC）的稳态mRNA水平和酶活性进行了测定。在肌肉、肌肉卫星细胞和肌管中，存在两种分子大小分别为2.05和1.75 kb的ODC mRNA转录本。Northern印迹分析表明，这些转录本是由于使用了不同的聚腺苷酸化位点而产生的。在孵化后第1天到第6天之间，这是这些动物肌肉快速生长的时期，两条品系的肌肉ODC mRNA水平达到峰值后，酶活性也达到峰值。在肉鸡品系中，显著更高的ODC mRNA水平和酶活性与快速生长的选择相关。这些结果与其他表明ODC是细胞生长主要因素的数据一致，并进一步证明它是生长的候选“性状基因”。
