大鼠肝脏中3-羟基-3-甲基戊二酰辅酶A还原酶、D位点结合蛋白（DBP）和丝氨酸脱水酶mRNA水平的昼夜变化发育模式。

Developmental patterns of diurnal variations in 3-hydroxy-3-methylglutaryl coenzyme A reductase, D-site binding protein (DBP), and serine dehydratase mRNA levels in rat liver.

作者信息

Ogawa H, Ansai Y

机构信息

Department of Biochemistry, Toyama Medical and Pharmaceutical University Faculty of Medicine, Japan.

出版信息

Comp Biochem Physiol B Biochem Mol Biol. 1995 Sep;112(1):39-47. doi: 10.1016/0305-0491(95)00053-b.

DOI:10.1016/0305-0491(95)00053-b

PMID:7584846

Abstract

Rats were housed under a 12-hr light (07:00-19:00)/12-hr dark cycle. Hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase mRNA appeared at 3 weeks of age, and diurnal variation was established at 5 weeks of age. Diurnal variation of the D-Site binding protein (DBP) mRNA was faintly seen 1 week after birth and became evident with age. Serine dehydratase mRNA level was high at 11:00 in the livers of rats at 2-3 days of age, and at 15:00 in those at 1-2 weeks of age. The rhythm became ambiguous at 3-4 weeks of age, but an adult-type rhythm with a peak at 19:00 was established at 5 weeks of age.

摘要

大鼠饲养于12小时光照（07:00 - 19:00）/12小时黑暗周期环境中。肝脏3-羟基-3-甲基戊二酰辅酶A还原酶mRNA在3周龄时出现，5周龄时建立昼夜节律变化。D位点结合蛋白（DBP）mRNA的昼夜节律变化在出生后1周隐约可见，并随年龄增长而明显。丝氨酸脱水酶mRNA水平在2 - 3日龄大鼠肝脏中于11:00时较高，在1 - 2周龄大鼠肝脏中于15:00时较高。这种节律在3 - 4周龄时变得不明显，但在5周龄时建立了以19:00为峰值的成年型节律。