通过钙离子和镁离子沉淀法从大鼠和猴小肠制备的刷状缘膜的比较研究。

Comparative study on brush border membranes prepared from rat and monkey small intestine by Ca2+ and Mg2+ precipitation.

作者信息

Ibrahim S A, Balasubramanian K A

机构信息

Wellcome Trust Research Laboratory, Department of Gastrointestinal Sciences, Christian Medical College & Hospital, Vellore, India.

出版信息

Comp Biochem Physiol B Biochem Mol Biol. 1995 Sep;112(1):65-9. doi: 10.1016/0305-0491(95)00057-f.

DOI:10.1016/0305-0491(95)00057-f

PMID:7584848

Abstract

Brush border membranes, prepared by Ca2+ or Mg2+ precipitation, from monkey or rat small intestine were compared for marker enzyme enrichment and lipid composition. Membranes prepared from rat intestine by Mg2+ precipitation were less pure in terms of their marker enzyme enrichment than Ca2+ precipitated membranes. Moreover, Mg2+ precipitated membranes were contaminated by basolateral membranes as evidenced by the enrichment of Na(+)-K+ ATPase. The content and composition of phospholipids were different in Mg2+ and Ca2+ precipitated membranes. The membranes prepared by the two methods from monkeys were identical in their marker enzyme enrichment and lipid composition and unlike those obtained from the rat, there was no basolateral membrane contamination.

摘要

通过钙离子或镁离子沉淀法制备的猴或大鼠小肠刷状缘膜，在标记酶富集和脂质组成方面进行了比较。用镁离子沉淀法从大鼠肠道制备的膜，就其标记酶富集而言，不如钙离子沉淀的膜纯净。此外，镁离子沉淀的膜被基底外侧膜污染，这可通过钠钾ATP酶的富集得到证明。镁离子和钙离子沉淀的膜中磷脂的含量和组成不同。用两种方法从猴子制备的膜在标记酶富集和脂质组成方面是相同的，与从大鼠获得的膜不同，不存在基底外侧膜污染。