Tachibana O, Nakazawa H, Lampe J, Watanabe K, Kleihues P, Ohgaki H
International Agency for Research on Cancer, Lyon, France.
Cancer Res. 1995 Dec 1;55(23):5528-30.
Fas/APO-1 (CD95) is an apoptosis-signaling receptor molecule on the surface of cells. To investigate the possible role of Fas during malignant transformation of glial cells, we analyzed the expression of Fas mRNA by reverse transcription-PCR in human astrocytic brain tumors. Expression was found in 1 of 4 (25%) juvenile pilocytic astrocytomas (WHO grade I), 1 of 9 (11%) low-grade astrocytomas (WHO grade II), 6 of 12 (50%) anaplastic astrocytomas (WHO grade III), and all of 9 glioblastomas (WHO grade IV). Thus, the frequency of Fas expression appears to correlate with the malignancy grade of astrocytomas. The soluble form of the Fas mRNA lacking the transmembrane domain was detected in one anaplastic astrocytoma and in two glioblastomas.
Fas/APO-1（CD95）是细胞表面的一种凋亡信号受体分子。为了研究Fas在胶质细胞恶性转化过程中可能发挥的作用，我们通过逆转录-聚合酶链反应（RT-PCR）分析了人类星形细胞脑肿瘤中Fas mRNA的表达情况。在4例（25%）青少年毛细胞型星形细胞瘤（世界卫生组织一级）中有1例检测到表达，9例（11%）低级别星形细胞瘤（世界卫生组织二级）中有1例，12例（50%）间变性星形细胞瘤（世界卫生组织三级）中有6例，以及所有9例胶质母细胞瘤（世界卫生组织四级）中均检测到表达。因此，Fas表达频率似乎与星形细胞瘤的恶性程度相关。在1例间变性星形细胞瘤和2例胶质母细胞瘤中检测到了缺乏跨膜结构域的Fas mRNA可溶性形式。
