利用黑曲霉的α-甘露糖苷酶通过逆水解法酶促合成甘露二糖和甘露三糖。

Enzymatic synthesis of mannobioses and mannotrioses by reverse hydrolysis using alpha-mannosidase from Aspergillus niger.

作者信息

Ajisaka K, Matsuo I, Isomura M, Fujimoto H, Shirakabe M, Okawa M

机构信息

Meiji Institute of Health Science, Meiji Milk Products Co., Ltd., Odawara, Japan.

出版信息

Carbohydr Res. 1995 Apr 30;270(2):123-30. doi: 10.1016/0008-6215(95)00015-l.

DOI:10.1016/0008-6215(95)00015-l

PMID:7585696

Abstract

Various manno-oligosaccharides including alpha-D-man-(1 --> 2)-D man and alpha-D-man-(1 --> 2)-alpha-D-man-(1 --> 2)-D-man were formed when a highly concentrated mannose solution was incubated in the presence of alpha-mannosidase from Aspergillus niger. alpha-D-Man-(1 --> 2)-D-man and alpha-D- man-(1 --> 2)-alpha-D-man-(1 --> 2)-D-man were isolated by activated carbon chromatography followed by high performance liquid chromatography using an amino-silica column. In addition to the above oligosaccharides, alpha-D-man-(1 --> 3)-D-man, alpha-D-man-(1 --> 6)-D-man, and alpha-D-man-(1 --> 2)-alpha-D-man-(1 -->6)-D-man were also isolated.

摘要

当在来自黑曲霉的α-甘露糖苷酶存在的情况下孵育高浓度甘露糖溶液时，会形成各种甘露寡糖，包括α-D-甘露糖-(1→2)-D-甘露糖和α-D-甘露糖-(1→2)-α-D-甘露糖-(1→2)-D-甘露糖。通过活性炭色谱法，然后使用氨基硅胶柱进行高效液相色谱法，分离出α-D-甘露糖-(1→2)-D-甘露糖和α-D-甘露糖-(1→2)-α-D-甘露糖-(1→2)-D-甘露糖。除了上述寡糖外，还分离出了α-D-甘露糖-(1→3)-D-甘露糖、α-D-甘露糖-(1→6)-D-甘露糖和α-D-甘露糖-(1→2)-α-D-甘露糖-(1→6)-D-甘露糖。