Indraccolo S, Mion M, Zamarchi R, Coppola V, Calderazzo F, Amadori A, Chieco-Bianchi L
Institute of Oncology, University of Padua, Italy.
Clin Immunol Immunopathol. 1995 Dec;77(3):253-61. doi: 10.1006/clin.1995.1151.
In this study we analyzed the behavior of a CD3+ T cell subpopulation lacking CD5 antigen expression in PBMC from HIV-1-infected patients. CD3+CD5- lymphocytes were greatly increased in peripheral blood of HIV-1+ patients, accounting for 20.6 +/- 9.9% of the total CD3+ cells, compared to seronegative individuals (5.5 +/- 3.2%). In both seropositive patients and controls, CD3+CD5- cells belonged to the CD8+ compartment; they were nonactivated, TCR alpha/beta+, naive lymphocytes, and in seronegative individuals preferentially expressed NK cell-associated markers, such as CD11b, CD16, CD56, and CD57. The phenotypic profile of this subset was slightly different in seropositive patients; while TCR expression and CD45RA/RO profile were comparable, CD11b and CD16 expression was lower compared to control figures, while CD56 expression was not changed, and CD57 expression was enhanced. Functional analysis of enriched CD3+CD8+CD5- cells showed an impaired ability to proliferate in response to mitogenic and antigenic stimuli; despite their NK-like phenotype, CD3+CD8+CD5- cells did not exert any NK cytotoxic activity, and only a lectin-dependent cytotoxic potential could be evidenced in this population. These results describe a novel alteration in the lymphocytes phenotypic profile during HIV-1 infection, involving a "transitional" population, which shares some properties of the T and of the NK cell lineage.
在本研究中，我们分析了HIV-1感染患者外周血单核细胞（PBMC）中缺乏CD5抗原表达的CD3⁺T细胞亚群的行为。与血清学阴性个体（5.5±3.2%）相比，HIV-1⁺患者外周血中CD3⁺CD5⁻淋巴细胞显著增加，占总CD3⁺细胞的20.6±9.9%。在血清阳性患者和对照组中，CD3⁺CD5⁻细胞均属于CD8⁺亚群；它们是非活化的、TCRα/β⁺、幼稚淋巴细胞，在血清学阴性个体中优先表达NK细胞相关标志物，如CD11b、CD16、CD56和CD57。该亚群的表型特征在血清阳性患者中略有不同；虽然TCR表达和CD45RA/RO谱相当，但与对照数据相比，CD11b和CD16表达较低，而CD56表达未改变，CD57表达增强。对富集的CD3⁺CD8⁺CD5⁻细胞的功能分析表明，其对有丝分裂原和抗原刺激的增殖能力受损；尽管它们具有NK样表型，但CD3⁺CD8⁺CD5⁻细胞未表现出任何NK细胞毒性活性，且在该群体中仅可证明有凝集素依赖性细胞毒性潜力。这些结果描述了HIV-1感染期间淋巴细胞表型特征的一种新改变，涉及一个“过渡性”群体，该群体兼具T细胞和NK细胞谱系的一些特性。
